Cloning and sequencing of the spherulin gene, the occlusion body major polypeptide of the Melolontha melolontha entomopoxvirus (MmEPV).

@article{Sanz1994CloningAS,
  title={Cloning and sequencing of the spherulin gene, the occlusion body major polypeptide of the Melolontha melolontha entomopoxvirus (MmEPV).},
  author={Paulo Sergio Sanz and J. C. Veyrunes and François Cousserans and Max Bergoin},
  journal={Virology},
  year={1994},
  volume={202 1},
  pages={449-57}
}
In the late stage of infection, virions of the Melolontha melolontha entomopoxvirus (MmEPV) are occluded into cytoplasmic paracrysalline proteinaceous occlusion bodies designated spherules (A. Amargier, C. Vago, G. Meynadier, 1964, Mikroskopie 19, 309-315). We have cloned and sequenced a 4-kpb DNA fragment of the MmEPV genome encompassing the spherule major protein gene named spherulin. The spherulin gene contains an open reading frame able to code for a 942-amino-acid (aa) polypeptide (MW 109… CONTINUE READING