Cloning and sequencing of the ribosomal RNA genes in maize: the 17S region.

@article{Messing1984CloningAS,
  title={Cloning and sequencing of the ribosomal RNA genes in maize: the 17S region.},
  author={Joachim Messing and John Carlson and G. Tim Hagen and Irwin Rubenstein and A E Oleson},
  journal={DNA},
  year={1984},
  volume={3 1},
  pages={31-40}
}
The maize genes for the 17S, 5.8S, and 26S ribosomal RNAs (rRNAs) are located on chromosome 6 and consist of 9-kb sequences repeated about 5,000-10,000 times per 2C. One of these sequences was isolated from a lambda library containing maize Eco RI genomic segments. The sequence of the small-subunit (17S) RNA was determined using the M13 shotgun-dideoxy sequencing approach. The maize sequence was compared with nuclear rRNAs from yeast, Xenopus, and rat. Using these sequences, it is possible to… CONTINUE READING

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