Cloning and sequencing of the gilthead sea bream estrogen receptor cDNA.

@article{MuozCueto1999CloningAS,
  title={Cloning and sequencing of the gilthead sea bream estrogen receptor cDNA.},
  author={Jos{\'e} A Mu{\~n}oz-Cueto and Elisabeth Burzawa-Gerard and Olivier Kah and Yves Valotaire and Farzad Pakdel},
  journal={DNA sequence : the journal of DNA sequencing and mapping},
  year={1999},
  volume={10 2},
  pages={75-84}
}
We report here the complete nucleotide sequence of a cDNA clone containing the full-coding sequence of the Sparus aurata estrogen receptor (ER) isolated from an expression library prepared from gilthead sea bream liver poly A+ RNA. The library was screened using a single strand rainbow trout ER cDNA probe, corresponding to the C-D domain. The cDNA sequence containing an insert of 2369 nucleotides was found to encode a protein of 579 amino acids. The 5'- and 3'-untranslated regions of the… CONTINUE READING

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