Cloning and sequencing of the gene which encodes the highly inducible acetamidase of Mycobacterium smegmatis.


The acetamidase of Mycobacterium smegmatis NCTC 8159 was purified, and the sequences of its amino-terminus and of two peptides obtained by proteolysis of the protein were obtained. A DNA fragment including the amidase structural gene was cloned in Escherichia coli, using oligonucleotide probes designed on the basis of the peptide sequences and a codon usage… (More)


5 Figures and Tables