Cloning and sequencing of the adenylate kinase gene (adk) of Escherichia coli.

@article{Brune1985CloningAS,
  title={Cloning and sequencing of the adenylate kinase gene (adk) of Escherichia coli.},
  author={Martin Brune and Renate Schumann and Fred Wittinghofer},
  journal={Nucleic acids research},
  year={1985},
  volume={13 19},
  pages={7139-51}
}
Adenylate kinase, the product of the adk locus in Escherichia coli K12, catalyzes the conversion of AMP and ATP to two molecules of ADP. The gene has been cloned by complementation of an adk temperature sensitive mutation. The DNA sequence of the complete coding region and of 5'- and 3'-untranslated regions were determined. The resulting protein sequence was found to contain several regions of high homology with cytosolic adenylate kinase of pig muscle (AK1), whose three-dimensional structure… CONTINUE READING