Cloning and sequencing of the Lactococcus lactis subsp. lactis groESL operon.

@article{Kim1993CloningAS,
  title={Cloning and sequencing of the Lactococcus lactis subsp. lactis groESL operon.},
  author={Sung Guk Kim and Carl A. Batt},
  journal={Gene},
  year={1993},
  volume={127 1},
  pages={121-6}
}
The operon (groESL) coding for the Lactococcus lactis subsp. lactis heat-shock proteins GroEL and GroES, has been isolated and its complete nucleotide (nt) sequence determined. A set of degenerate PCR primers, deduced from amino acids which are conserved in a number of prokaryotic GroELs, were synthesized and used to amplify a 957-bp fragment. This PCR fragment was used as a probe to isolate a 5.0-kb EcoRI chromosomally derived fragment. A region of this 5.0-kb EcoRI fragment was sequenced and… CONTINUE READING
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