Cloning and sequencing of pel gene responsible for CMCase activity from Erwinia chrysanthemi PY35.

Abstract

The phytopathogenic bacterium Erwinia chrysanthemi secretes multiple isozymes of plant cell wall disrupting enzymes such as pectate lyase and endoglucanase. We cloned genomic DNA from Erwinia chrysanthemi PY35. One of the E. coli XL1-Blue clones contained a 5.1-kb BamHI fragment and hydrolyzed carboxymethyl cellulose and polygalacturonic acid. By subsequent… (More)

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@article{Park2000CloningAS, title={Cloning and sequencing of pel gene responsible for CMCase activity from Erwinia chrysanthemi PY35.}, author={S. R. Park and S. J. Cho and H. D. Yun}, journal={Bioscience, biotechnology, and biochemistry}, year={2000}, volume={64 5}, pages={925-30} }