Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22.

@article{TsaiPflugfelder1988CloningAS,
  title={Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22.},
  author={M. Tsai-Pflugfelder and L. Liu and A. Liu and K. M. Tewey and J. Whang-peng and T. Knutsen and K. Huebner and C. Croce and J. Wang},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1988},
  volume={85 19},
  pages={
          7177-81
        }
}
Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage lambda was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a lambda gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific… Expand
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