Cloning and sequencing of a gene encoding a 2S albumin seed storage protein precursor from English walnut (Juglans regia), a major food allergen.

@article{Teuber1998CloningAS,
  title={Cloning and sequencing of a gene encoding a 2S albumin seed storage protein precursor from English walnut (Juglans regia), a major food allergen.},
  author={Suzanne S. Teuber and Abhaya M Dandekar and Wambugu Peterson and Clay L Sellers},
  journal={The Journal of allergy and clinical immunology},
  year={1998},
  volume={101 6 Pt 1},
  pages={807-14}
}
BACKGROUND Walnuts rank third in per capita consumption of tree nuts in the United States and can be associated with systemic IgE-mediated reactions in some individuals. OBJECTIVE The objectives of the study were to clone a gene encoding one of the major food allergens in the walnut kernel and to characterize the recombinant allergen. METHODS A cDNA expression library in the lambda vector Uni-ZAP, which was prepared from walnut somatic embryos, was screened by using a patient's sera that… CONTINUE READING
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