Cloning and sequencing of a cDNA encoding the major storage proteins of Theobroma cacao

@article{Spencer1992CloningAS,
  title={Cloning and sequencing of a cDNA encoding the major storage proteins of Theobroma cacao},
  author={Margaret E. Spencer and Rachel Hodge},
  journal={Planta},
  year={1992},
  volume={186},
  pages={567-576}
}
The major storage proteins, polypeptides of 31 and 47 kilodaltons (kDa), from the seeds of cocoa (Theobroma cacao L.), have been identified and partially purified by preparative gel electrophoresis. The polypeptides were both N-terminally blocked, but some N-terminal amino-acid sequence was obtained from a cyanogen bromide peptide common to both polypeptides, permitting the construction of an oligonucleotide probe. This probe was used to isolate the corresponding copy-DNA (cDNA) clone from a… CONTINUE READING