Cloning and sequencing of a Candida albicans catalase gene and effects of disruption of this gene.

@article{Wysong1998CloningAS,
  title={Cloning and sequencing of a Candida albicans catalase gene and effects of disruption of this gene.},
  author={Deborah R Wysong and L Christin and Alan M. Sugar and Phillips W. Robbins and Richard D. Diamond},
  journal={Infection and immunity},
  year={1998},
  volume={66 5},
  pages={1953-61}
}
Catalase plays a key role as an antioxidant, protecting aerobic organisms from the toxic effects of hydrogen peroxide, and in some cases has been postulated to be a virulence factor. To help elucidate the function of catalase in Candida albicans, a single C. albicans-derived catalase gene, designated CAT1, was isolated and cloned. Degenerate PCR primers based on highly conserved areas of other fungal catalase genes were used to amplify a 411-bp product from genomic DNA of C. albicans ATCC 10261… CONTINUE READING