Cloning and sequence of the groESL heat-shock operon of Pasteurella multocida.

@article{Love1995CloningAS,
  title={Cloning and sequence of the groESL heat-shock operon of Pasteurella multocida.},
  author={B C Love and Lori M. Hansen and Dwight C Hirsh},
  journal={Gene},
  year={1995},
  volume={166 1},
  pages={179-80}
}
By using degenerate oligodeoxyribonucleotide primers for conserved regions of groEL, a 0.6-kb fragment of Pasteurella multocida genomic DNA was amplified using PCR. The amplified fragment was then used as a probe to isolate a genomic fragment containing the entire GroESL operon. The isolated genomic fragment was found to contain two open reading frames, the… CONTINUE READING