Cloning and sequence analysis of two copies of a 23S rRNA gene from Helicobacter pylori and association of clarithromycin resistance with 23S rRNA mutations.

@article{Taylor1997CloningAS,
  title={Cloning and sequence analysis of two copies of a 23S rRNA gene from Helicobacter pylori and association of clarithromycin resistance with 23S rRNA mutations.},
  author={Diane E. Taylor and Zhongming Ge and D Purych and Ta-Chun Lo and Koji Hiratsuka},
  journal={Antimicrobial agents and chemotherapy},
  year={1997},
  volume={41 12},
  pages={2621-8}
}
In this study, two identical copies of a 23S-5S gene cluster, which are separately situated within the Helicobacter pylori UA802 chromosome, were cloned and sequenced. Comparison of the DNA sequence of the H. pylori 23S rRNA gene with known sequences of other bacterial 23S rRNA genes indicated that the H. pylori UA802 23S rRNA genes are closely related to those of Campylobacter spp. and therefore belong in the proposed Proteobacteria subdivision. The 5'-terminal nucleotide T or A of the 23S… CONTINUE READING

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The transformation frequency for Cla(r ) and Ery(r ) was found to be approximately 2 x 10(-6 ) transformants per viable cell , and the MICs of both clarithromycin and erythromycin for the Cla(r ) Ery(r ) mutants were equal to those for the donor isolate .
Cross - resistance to clarithromycin and erythromycin , which was transferred by natural transformation from the Cla(r ) Ery(r ) donor strain H. pylori E to the Cla(s ) Ery(s ) recipient strain H. pylori UA802 , was associated with an single A - to - G transition mutation at position 2142 of both copies of the 23S rRNA in UA802 Cla(r ) Ery(r ) mutants .
The transformation frequency for Cla(r ) and Ery(r ) was found to be approximately 2 x 10(-6 ) transformants per viable cell , and the MICs of both clarithromycin and erythromycin for the Cla(r ) Ery(r ) mutants were equal to those for the donor isolate .
Cross - resistance to clarithromycin and erythromycin , which was transferred by natural transformation from the Cla(r ) Ery(r ) donor strain H. pylori E to the Cla(s ) Ery(s ) recipient strain H. pylori UA802 , was associated with an single A - to - G transition mutation at position 2142 of both copies of the 23S rRNA in UA802 Cla(r ) Ery(r ) mutants .
The transformation frequency for Cla(r ) and Ery(r ) was found to be approximately 2 x 10(-6 ) transformants per viable cell , and the MICs of both clarithromycin and erythromycin for the Cla(r ) Ery(r ) mutants were equal to those for the donor isolate .
Cross - resistance to clarithromycin and erythromycin , which was transferred by natural transformation from the Cla(r ) Ery(r ) donor strain H. pylori E to the Cla(s ) Ery(s ) recipient strain H. pylori UA802 , was associated with an single A - to - G transition mutation at position 2142 of both copies of the 23S rRNA in UA802 Cla(r ) Ery(r ) mutants .
The transformation frequency for Cla(r ) and Ery(r ) was found to be approximately 2 x 10(-6 ) transformants per viable cell , and the MICs of both clarithromycin and erythromycin for the Cla(r ) Ery(r ) mutants were equal to those for the donor isolate .
Cross - resistance to clarithromycin and erythromycin , which was transferred by natural transformation from the Cla(r ) Ery(r ) donor strain H. pylori E to the Cla(s ) Ery(s ) recipient strain H. pylori UA802 , was associated with an single A - to - G transition mutation at position 2142 of both copies of the 23S rRNA in UA802 Cla(r ) Ery(r ) mutants .
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