Cloning and sequence analysis of the putative rifamycin polyketide synthase gene cluster from Amycolatopsis mediterranei.

@article{Schupp1998CloningAS,
  title={Cloning and sequence analysis of the putative rifamycin polyketide synthase gene cluster from Amycolatopsis mediterranei.},
  author={Thomas Schupp and C. Toupet and Nora Engel and Stephen P Goff},
  journal={FEMS microbiology letters},
  year={1998},
  volume={159 2},
  pages={
          201-7
        }
}
The 54-kbp Type I polyketide synthase gene cluster, most probably involved in rifamycin biosynthesis by Amycolatopsis mediterranei, was cloned in E. coli and completely sequenced. The DNA encodes five closely packed, very large open reading frames reading in one direction. As expected from the chemical structure of rifamycins, ten polyketide synthase modules and a CoA ligase domain were identified in the five open reading frames which contain one to three polyketide synthase modules each. The… CONTINUE READING
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