Cloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene.

@article{Zhu2015CloningAS,
  title={Cloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene.},
  author={Hui Zhu and Shengzhou Zhang and Yongkang Zhou and Chaolin Wang and Xiaobing Wu},
  journal={Genetics and molecular research : GMR},
  year={2015},
  volume={14 4},
  pages={12159-67}
}
β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequence analysis showed that this fragment contained the complete coding sequence of the β-actin gene (1128 bp), encoding 375 amino acids. The amino acid sequence of β-actin is highly… CONTINUE READING
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