Cloning and sequence analysis of the Schistosoma mansoni membrane glycoprotein antigen gene GP22.

@article{ElSherbeini1991CloningAS,
  title={Cloning and sequence analysis of the Schistosoma mansoni membrane glycoprotein antigen gene GP22.},
  author={Mohamed El-Sherbeini and Naasa Ramadan and Keith A. Bostian and Paul Mark Knopf},
  journal={Molecular and biochemical parasitology},
  year={1991},
  volume={49 1},
  pages={83-98}
}
A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hybridization with a lambda gt11-40 oligonucleotide probe. In the 1.37 kb of genomic DNA sequenced, an open reading frame of 182 amino acids was identified on the strand corresponding… CONTINUE READING