Cloning and sequence analysis of a novel xylanase gene, Auxyn10A, from Aspergillus usamii

@article{Wang2011CloningAS,
  title={Cloning and sequence analysis of a novel xylanase gene, Auxyn10A, from Aspergillus usamii},
  author={Junqing Wang and Huimin Zhang and Minchen Wu and Cunduo Tang},
  journal={Biotechnology Letters},
  year={2011},
  volume={33},
  pages={1029-1038}
}
A full-length cDNA sequence, encoding a novel endo-1,4-β-d-xylanase (AuXyn10A) of Aspergillus usamii, was obtained by using rapid amplification of cDNA ends (RACE) methods and cloned into the pUCm-T vector, followed by DNA sequencing. The cDNA gene, designated as Auxyn10A, is 1,235 bp in length harboring 5′- and 3′-non-encoding regions, as well as an ORF of 984 bp that encodes a 19-aa signal peptide, a 6-aa propeptide and a 302-aa mature peptide with a calculated MW of 32,756 Da. The AuXyn10A… CONTINUE READING