Cloning and sequence analysis of a novel hemolysin gene (vllY) from Vibrio vulnificus.

@article{Chang1997CloningAS,
  title={Cloning and sequence analysis of a novel hemolysin gene (vllY) from Vibrio vulnificus.},
  author={Tsai-Rong Chang and Yin Ching Chuang and Jinu Huang Su and Ming Chung Chang},
  journal={Applied and environmental microbiology},
  year={1997},
  volume={63 10},
  pages={3851-7}
}
A gene (vllY) encoding a novel hemolysin of Vibrio vulnificus CKM-1 has been cloned and sequenced. When the vllY gene was expressed in minicells, a unique peptide of approximately 40 kDa was identified. Subcellular fractionation of Escherichia coli cells carrying the vllY gene indicated that the VllY protein was distributed in both the cytoplasmic and the periplasmic fractions, with the notable ability to appear in the latter compartment. Nucleotide sequence analysis predicted a single open… CONTINUE READING

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