Cloning and sequence analysis of a protease-encoding gene from the marine bacterium Alteromonas sp. strain O-7.

@article{Tsujibo1996CloningAS,
  title={Cloning and sequence analysis of a protease-encoding gene from the marine bacterium Alteromonas sp. strain O-7.},
  author={Hiroshi Tsujibo and Kenji Miyamoto and Kunihito Tanaka and Yoshihiko Kaidzu and Chiaki Imada and Yoshiro Okami and Yoshihiko Inamori},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1996},
  volume={60 8},
  pages={1284-8}
}
The gene (aprI) encoding alkaline serine protease (AprI; subtilase) from Alteromonas sp. strain O-7 was cloned and sequenced. The nucleotide sequence of aprI has been identified. The deduced amino acid sequence indicated that aprI codes for a precursor of 715 amino acids and the precursor is composed of four regions including a signal peptide, an N-terminal pro-region, a mature protease region and a C-terminal extension region of 215 amino acids as previously described for aprII [H. Tsujibo et… CONTINUE READING