Cloning and physical characterization of katE and katF required for catalase HPII expression in Escherichia coli.

@article{Mulvey1988CloningAP,
  title={Cloning and physical characterization of katE and katF required for catalase HPII expression in Escherichia coli.},
  author={Michael R. Mulvey and P A Sorby and Barbara Triggs-Raine and Peter C Loewen},
  journal={Gene},
  year={1988},
  volume={73 2},
  pages={337-45}
}
Two genes, katE and katF, affecting the synthesis of catalase HPII in Escherichia coli, have been cloned. The multistep cloning protocol involved: screening for the tet gene in a transposon interrupting the genes, selecting DNA adjacent to the transposon, and using it to probe a library of wild-type DNA to select clones from which katE and katF were subcloned into pAT153. The clones were physically characterized and the presence of the genes confirmed by complementation of their respective… CONTINUE READING

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