Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast.

@article{Hishinuma1984CloningAN,
  title={Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast.},
  author={Fumio Hishinuma and Kozo Nakamura and Keiko Hirai and R Nishizawa and Norio Gunge and Tatsuya Maeda},
  journal={Nucleic acids research},
  year={1984},
  volume={12 19},
  pages={7581-97}
}
The linear DNA killer plasmids (pGKL1 and pGKL2) isolated from a Kluyveromyces lactis killer strain are also maintained and expressed its killer character in Saccharomyces cerevisiae. After these killer plasmid DNAs isolated from S. cerevisiae were treated with alkali, four terminal fragments from each plasmid DNAs were cloned separately. Using these and other cloned DNA fragments, the terminal nucleotide sequences of pGKL2 and the complete nucleotide sequence of pGKL1 were determined. The… CONTINUE READING

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