Cloning and nucleotide sequences of crotamine genes.

  title={Cloning and nucleotide sequences of crotamine genes.},
  author={L. A. Smith and J. W. Schmidt},
  journal={Toxicon : official journal of the International Society on Toxinology},
  volume={28 5},
A cDNA library containing snake toxin genes was constructed in bacteriophage lambda by using mRNA isolated from the glands of the South American rattlesnake, Crotalus durissus terrificus. The first high-density screening of 400,000 plaques for crotamine-containing genes yielded over 800 positives when a labeled cDNA probe with sequence homology to crotamine was used. Four of these clones with insert sizes from 270 to 400 base pairs were chosen and their inserts subcloned into pGEM-3Z and… CONTINUE READING
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