Cloning and nucleotide sequence of the gene coding for citrate synthase from a thermotolerant Bacillus sp.

Abstract

The structural gene coding for citrate synthase from the gram-positive soil isolate Bacillus sp. strain C4 (ATCC 55182) capable of secreting acetic acid at pH 5.0 to 7.0 in the presence of dolime has been cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking citrate synthase. The nucleotide sequence of the entire… (More)

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Cite this paper

@article{Schendel1992CloningAN, title={Cloning and nucleotide sequence of the gene coding for citrate synthase from a thermotolerant Bacillus sp.}, author={Frederick J Schendel and Phyllis August and C. R. Anderson and Ronald Hanson and M C Flickinger}, journal={Applied and environmental microbiology}, year={1992}, volume={58 1}, pages={335-45} }