Cloning and nucleotide sequence of the gene coding for enzymatically active fragments of the Bacillus polymyxa beta-amylase.

Abstract

The gene encoding beta-amylase was cloned from Bacillus polymyxa 72 into Escherichia coli HB101 by inserting HindIII-generated DNA fragments into the HindIII site of pBR322. The 4.8-kilobase insert was shown to direct the synthesis of beta-amylase. A 1.8-kilobase AccI-AccI fragment of the donor strain DNA was sufficient for the beta-amylase synthesis… (More)

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@article{Kawazu1987CloningAN, title={Cloning and nucleotide sequence of the gene coding for enzymatically active fragments of the Bacillus polymyxa beta-amylase.}, author={Tetsu Kawazu and Y. Nakanishi and Naofumi Uozumi and T. Sasaki and Hiroshi Yamagata and Norihiro Tsukagoshi and Shigezo Udaka}, journal={Journal of bacteriology}, year={1987}, volume={169 4}, pages={1564-70} }