Cloning and nucleotide sequence of the acid protease-encoding gene (pepA) from Aspergillus oryzae.

@article{Gomi1993CloningAN,
  title={Cloning and nucleotide sequence of the acid protease-encoding gene (pepA) from Aspergillus oryzae.},
  author={Kazunori Gomi and Kentaro Arikawa and Nobuo Kamiya and Katsuhiko Kitamoto and Chieko Kumagai},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1993},
  volume={57 7},
  pages={1095-100}
}
We have cloned a genomic DNA sequence encoding the acid protease (PEPA) from Aspergillus oryzae using a 0.6-kb fragment as a probe. This fragment was amplified by the polymerase chain reaction (PCR) using oligonucleotide primers designed from the partial amino acid sequences of peptide fragments of the purified protein. Nucleotide sequencing analysis has shown that the cloned gene (designated pepA) encodes 404 amino acid residues and contains 3 putative introns ranging in length from 50 to 61… CONTINUE READING