Cloning and nucleotide sequence of the KHS killer gene of Saccharomyces cerevisiae.

@article{Goto1990CloningAN,
  title={Cloning and nucleotide sequence of the KHS killer gene of Saccharomyces cerevisiae.},
  author={Kanako Goto and Hiroo Fukuda and K Kichise and Kenji Kitano and Saburo Hara},
  journal={Agricultural and biological chemistry},
  year={1990},
  volume={55 8},
  pages={1953-8}
}
A 5.3-kbp fragment of the KHS gene was cloned from a genomic bank of Saccharomyces cerevisiae No. 115 constructed with an E. coli as the host and YEp13 as the vector. A non-killer yeast strain was transformed to a killer strain with the multi-copy vector containing the KHS gene, and the transformant could secrete 3-4 times more killer toxin into culture… CONTINUE READING