Cloning and nucleotide sequence of a cellulase gene, casA, from an alkalophilic Streptomyces strain

@inproceedings{Ryozo1988CloningAN,
  title={Cloning and nucleotide sequence of a cellulase gene, casA, from an alkalophilic Streptomyces strain},
  author={Nakai Ryozo and Horinouchi Sueharu and Beppu Teruhiko},
  year={1988}
}
Abstract A gene encoding an endo-type semi-alkaline cellulase was cloned from an alkalophilic Streptomyces strain in Streptomyces lividans , and its nucleotide sequence was determined. Downstream from the transcriptional start point, which was determined by high-resolution S1 mapping, an open reading frame of 388 amino acids (aa) was present. The N-terminal amino acid sequence of the mature enzyme determined by an Edman degradation procedure suggested that the cellulase had an extraordinarily… CONTINUE READING

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