Cloning and nucleotide sequence of a cDNA encoding ammodytoxin A, the most toxic phospholipase A2 from the venom of long-nosed viper (Vipera ammodytes).


A venom gland cDNA library was constructed in pUC9 and screened with a mixed oligonucleotide probe deduced from the unique Glu-4 to Ile-9 region of ammodytoxins. Twenty-one strongly positive clones were found by hybridization of about 5000 bacterial colonies, nine of them with the inserts encoding ammodytoxin A. The cDNA for ammodytoxin A encodes a 122… (More)


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