Cloning and molecular analysis of the poly(3-hydroxybutyric acid) biosynthetic genes of Thiocystis violacea

@article{Liebergesell2004CloningAM,
  title={Cloning and molecular analysis of the poly(3-hydroxybutyric acid) biosynthetic genes of Thiocystis violacea},
  author={Matthias Liebergesell and Alexander Steinb{\"u}chel},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={38},
  pages={493-501}
}
From a genomic library of Thiocystis violaceae strain 2311 in λL47, two adjacent EcoRI restriction fragments of 5361 base pairs (bp) and of 1978 bp were cloned. The 5361-bp EcoRI restriction fragment hybridized with a DNA fragment harbouring the Alcaligenes eutrophus poly(3-hydroxyalkanoate) (PHA) synthase operon (phbCAB) and restored the ability to synthesize and accumulate PHA in PHA-negative mutants derived from A. eutrophus. The nucleotide sequence analysis of both fragments revealed five… 

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The results of this study make available a phylogenetically diverse type III phaC and phaE genes, and confirm through kanamycin selection pressure the existence of multiple PHA biosynthesis systems in R. eutropha.

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Application of enzymatically synthesized short-chain-length hydroxy fatty acid coenzyme A thioesters for assay of polyhydroxyalkanoic acid synthases

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