Cloning and mapping of the replication origin of Escherichia coli.

  title={Cloning and mapping of the replication origin of Escherichia coli.},
  author={Shinsuke Yasuda and Yoshiko Hirota},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={74 12},
The replication origin of Escherichia coli has been cloned on a nonreplicating DNA fragment coding for ampicillin resistance. This recombinant DNA, named pSY211, replicates depending on the presence of the replication origin and can be recovered as a closed circular plasmid DNA of 10.7 megadaltons (Mdal). A restriction map has been constructed. EcoRI cleaves pSY211 into two fragments: one is the ampicillin fragment of 4.5 Mdal and the other is a chromosomal fragment of 6 Mdal and contains the… CONTINUE READING
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