Cloning and heterologous expression of nitrate reductase genes from Dunaliella salina

@article{Xie2007CloningAH,
  title={Cloning and heterologous expression of nitrate reductase genes from Dunaliella salina},
  author={Hua Xie and Peirong Xu and Yanlong Jia and Jie Li and Yumin Lu and Lexun Xue},
  journal={Journal of Applied Phycology},
  year={2007},
  volume={19},
  pages={497-504}
}
A cDNA corresponding to the nitrate reductase (NR) gene from Dunaliella salina was isolated by RT-PCR and (5′/3′)-RACE techniques. The full-length cDNA sequence of 3,694 bp contained an open reading frame of 2,703 bp encoding 900 amino acids, a 5′-untranslated region of 151 bp and a 3′-untranslated sequence of 840 bp with a poly (A) tail. The putative gene product exhibited 78%, 65%, 59% and 50% identity in amino acid sequence to the corresponding genes of Dunaliella tertiolecta, Volvox carteri… CONTINUE READING

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