Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization

Abstract

To clone, express and characterize uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD) from Lactobacillus reuteri. Some strains of Lb. reuteri produce cobalamin (vitamin B12). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy l-methionine uroprophyrinogen III methyltransferase/synthase (CobA/HemD), a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols.

DOI: 10.1007/s10529-011-0609-5

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Cite this paper

@article{Vannini2011CloningAH, title={Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization}, author={Ver{\'o}nica Vannini and Adriana Pinheiro Martinelli Rodriguez and Jos{\'e} L. del Castillo-Pardo de Vera and Graciela Font de Valdez and Mar{\'i}a P{\'i}a Taranto and Fernando J. M. Sesma}, journal={Biotechnology Letters}, year={2011}, volume={33}, pages={1625-1632} }