Cloning and genetic characterization of the flagellum subunit gene (flaA) of Legionella pneumophila serogroup 1.

@article{Heuner1995CloningAG,
  title={Cloning and genetic characterization of the flagellum subunit gene (flaA) of Legionella pneumophila serogroup 1.},
  author={Klaus Heuner and L Bender-Beck and B C Brand and Paul Christian L{\"u}ck and Ken H. Mann and Reinhard Marre and Michael Ott and Joerg Hacker},
  journal={Infection and immunity},
  year={1995},
  volume={63 7},
  pages={2499-507}
}
The gene flaA, encoding the flagellum subunit protein of Legionella pneumophila serogroup 1, has been isolated from an expression library of L. pneumophila isolate Corby in Escherichia coli K-12 by using an antiflagellin specific polyclonal antiserum. DNA sequence analysis of the flaA gene revealed the presence of a 1,428-bp open reading frame encoding a protein of 475 amino acids with an apparent molecular mass of 48 kDa that is expressed independently of an E. coli vector promoter. Peptide… CONTINUE READING
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