Cloning and functional expression of dipeptidyl peptidase IV from the ruminal bacterium Prevotella albensis M384(T).

@article{Walker2003CloningAF,
  title={Cloning and functional expression of dipeptidyl peptidase IV from the ruminal bacterium Prevotella albensis M384(T).},
  author={Nicola D. Walker and N. R. Mcewan and Robert J. Wallace},
  journal={Microbiology},
  year={2003},
  volume={149 Pt 8},
  pages={2227-34}
}
Ruminal bacteria of the genus Prevotella play a crucial role in peptide breakdown in the rumen, a component of protein catabolism that leads to the inefficient use of dietary protein by ruminant animals. This is the first report of the cloning of a peptidase gene from a ruminal bacterium. Part of the dipeptidyl peptidase type IV (DPP-IV) gene from Prevotella albensis M384(T) was cloned using degenerate primers designed from conserved regions found within other known DPP-IV sequences. Flanking… CONTINUE READING

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