Cloning and functional expression of a cDNA encoding coffee bean alpha-galactosidase.

@article{Zhu1994CloningAF,
  title={Cloning and functional expression of a cDNA encoding coffee bean alpha-galactosidase.},
  author={Aiping Zhu and Jay M Goldstein},
  journal={Gene},
  year={1994},
  volume={140 2},
  pages={227-31}
}
Purified coffee bean alpha-galactosidase (alpha Gal) has been used for removing terminal alpha-galactose residues from the glyco-conjugates at the red cell surface, in studies of blood group conversion. Here, we report the isolation and sequence of the full-length clone for coffee bean alpha Gal by using the polymerase chain reaction (PCR) and rapid… CONTINUE READING