Cloning and functional expression in Saccharomyces cereviae of a K+ transporter, AlHAK, from the graminaceous halophyte, Aeluropus littoralis

Abstract

High-affinity K+ transporters play an important role in K+ absorption of plants. We isolated a HAK gene from Aeluropus littoralis, a graminaceous halophyte. The amino acid sequence of AlHAK showed high homology with HAK transporters obtained from Oryza sativa (82%) and Hordeum vulgare (82%). When expressed in Saccharomyces cereviae WΔ3, AlHAK performed high-affinity K+ uptake with a Km value of 8 μM, and the growth of transformants was dramatically inhibited by 150 mM Rb+ and 150 mM Cs+ but less affected by 300 mM Na+. AlHAK may thus improve the capacity of plants to maintain a high cytosolic K+/Na+ ratio at high salinity.

DOI: 10.1007/s10529-007-9484-5

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Cite this paper

@article{Su2007CloningAF, title={Cloning and functional expression in Saccharomyces cereviae of a K+ transporter, AlHAK, from the graminaceous halophyte, Aeluropus littoralis}, author={Qiao Su and S. Q. Feng and Lijia An and Gaohua Zhang}, journal={Biotechnology Letters}, year={2007}, volume={29}, pages={1959-1963} }