Cloning and functional characterization of the human 5‐HT2B serotonin receptor

  title={Cloning and functional characterization of the human 5‐HT2B serotonin receptor},
  author={Karin Schmuck and Christoph Ullmer and Peter Engels and Hermann Lübbert},
  journal={FEBS Letters},

Gene Structure, Expression, and 5-HT2B Receptor Signaling

The complexity of its signaling in relation with its expression, including in various types of cancer cells, is developed and discussed.

The pharmacology and distribution of human 5‐hydroxytryptamine2B (5‐HT2b) receptor gene products: comparison with 5‐HT2a and 5‐HT2c receptors

These findings confirm the sequence of the human 5‐HT2B receptor and they demonstrate that the receptor has a widespread tissue distribution and suggest that there are differences in ligand affinities between different species homologues of the receptor.

Development and Characterization of Monoclonal Antibodies Specific to the Serotonin 5-HT2A Receptor

  • C. WuE. Yoder S. Singh
  • Biology
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 1998
The development and characterization of MAbs specific to the serotonin 5-HT2A receptor are reported, which showed differential staining in various regions of the rat brain, varying from regions with no staining to regions of intense reactivity.

Localization of the gene for the human serotonin 5-HT(2B) receptor to chromosome 2.

A 483 bp Bgl II/Sal I repeat free fragment was isolated from the vector containing the human serotonin 5-HT(2B) receptor and revealed 100 percent concordance with location of the gene for this 5- HT receptor variant at chromosome 2.



Structure of the mouse 5‐HT 1c 5‐HT 2 and stomach fundus serotonin receptor genes

By analysis of the mouse 5-HT1C receptor gene, it is found that its coding region contains three introns, which is unique and distinguishes these receptors from other members of the family of receptors coupled to G-proteins.

Cloning of the human serotonin 5-HT2 and 5-HT1C receptor subtypes.

Cloning and functional characterization of the rat stomach fundus serotonin receptor.

A DNA segment homologous to the third exons of the serotonin 1C and 2 receptor genes was isolated from a mouse genomic library and the pharmacological profile resembled that described for the serotonin‐stimulated contraction of the stomach fundus.

The 5HT2 receptor defines a family of structurally distinct but functionally conserved serotonin receptors.

A rat brain 5HT2 serotonin receptor cDNA is isolated by virtue of its homology with the 5HT1c receptor and RNA blotting shows that these two receptor subtypes are differentially expressed in the central nervous system.

Pharmacological characteristics of the newly cloned rat 5-hydroxytryptamine2F receptor.

The affinity of a compound for the 5-HT2F receptor at 37 degrees versus 0 degree was shown to be useful for predicting agonist or antagonist activity, and information is provided about some of the structural requirements for the affinity of certain tryptamines at the 4-HT/1C receptor family.

Human serotonin1B receptor expression in Sf9 cells: phosphorylation, palmitoylation, and adenylyl cyclase inhibition.

Analysis of the primary protein structure of the human serotonin1B (5-HT1B) receptor reveals consensus sites for phosphorylation and a putative site for palmitoylation, and a c-myc epitope-tagged 5- HT1B receptor is expressed in Sf9 cells to investigate posttranslational modifications.

Expression cloning of a rat brain somatostatin receptor cDNA.

The deduced amino acid sequence of the receptor displays sequence and structural homology to the family of G-protein-coupled receptors, and it is concluded that a rat brain SRIF receptor cDNA is isolated.

cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes.

A strategy for the cloning of neurotransmitter-receptor and ion-channel cDNAs that is based on electrophysiological assays of mRNA-injected Xenopus oocytes is described, and the isolation of a serotonin receptor cDNA clone from a mouse choroid plexus papilloma is demonstrated.

5-HT1c receptor is a prominent serotonin receptor subtype in the central nervous system.

The 5-HT1c receptor is a prominent but poorly characterized central subclass of serotonin (5-HT) receptor that is expressed in a wide variety of cortical and subcortical neurons.