Cloning and functional characterisation of the human TRH receptor

  title={Cloning and functional characterisation of the human TRH receptor},
  author={Sarah M. Duthie and Philip L. Taylor and L A Anderson and J V Cook and Karin Ann Eidne},
  journal={Molecular and Cellular Endocrinology},

TRH acts as a multifunctional hypophysiotropic factor in vertebrates.

Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis.

expression in Xenopus oocytes and HEK-293 cells indicates that the three Xenopus TRHRs are fully functional and are coupled to the inositol phosphate/calcium pathway.

Thyrotropin‐Releasing Hormone

TRH functions as a neurotransmitter and neuromodulator and is involved in various neurobehavioral functions, including sleep, anxiety, depression, learning, and memory.

Cloning and characterization of the chicken thyrotropin-releasing hormone receptor.

These findings show that there have been considerable evolutionary constraints on TRH receptor structure and function and provide a useful tool for further studies on the roles of TRH in avian growth and TSH regulation.

Human TRH-degrading ectoenzyme cDNA cloning, functional expression, genomic structure and chromosomal assignment.

All of these data further the notion that the TRH-DE is not an ordinary enzyme but a specific neuropeptidase that has been highly conserved among species.

Cloning of two thyrotropin-releasing hormone receptor subtypes from a lower vertebrate (Catostomus commersoni): functional expression, gene structure, and evolution.

The analysis of the gene structure of ccTRH-R and the amino acid sequence comparisons of mammalian and teleost TRH- R of both subtypes suggest that TRH receptors have been highly conserved during the course of vertebrate evolution.

Biochemical and physiological insights into TRH receptor-mediated signaling

This review summary summarizes what has been learned to date about TRH receptor-mediated signaling, including some previously undiscussed information, and point to future directions in TRH research that may offer new insights into the molecular mechanisms of TRH receptors-triggered actions and possible ways to modulate TRh receptor- mediated signaling.

Thyrotropin-releasing hormone receptors -- similarities and differences.

Although they both can be stimulated by TRH to similar maximal signaling levels, TRh-R2 exhibits higher basal signaling activity and is more rapidly internalized than TRH-R1, and these differences in signaling and internalization properties are probably important in the distinct parts that TRH/R2 may play in mammalian physiology.



Functional expression and molecular characterization of the thyrotrophin-releasing hormone receptor from the rat anterior pituitary gland.

The rat TRH-R protein showed considerable homology with that of the mouse, except for a deletion of 232 bp in the 3'-coding region, which did not appear to affect the functional characteristics of the receptor, as shown by electrophysiological studies with Xenopus oocytes and by transfection of the cDNA into COS-7 cells.

Cloning and expression of the thyrotropin-releasing hormone receptor from GH3 rat anterior pituitary cells.

Full functionality of the predicted 412-amino-acid receptor protein was demonstrated by functional expression of cell surface receptors in Xenopus oocytes after both cytoplasmic injection of sense RNA transcribed in vitro from this cDNA and nuclear injection of the cDNA under the control of the Herpes simplex virus thymidine kinase promoter.

Molecular cloning of a complementary deoxyribonucleic acid encoding the thyrotropin-releasing hormone receptor and regulation of its messenger ribonucleic acid in rat GH cells.

It is concluded that the rat TRH receptor (rTRHR) shares high primary sequence similarity to the mouse thyrotrope TRHR, but the rTRHR has an extension of 19 amino acids at its carboxy-terminus, which is lacking in the mTRHR.

Characterization of the gonadotrophin-releasing hormone receptor in alpha T3-1 pituitary gonadotroph cells.

Results show that the GnRH receptors expressed in alpha T3-1 cells are coupled to the phosphatidylinositol second messenger pathway via a specific G-protein.

Thyrotropin‐Releasing hormone gene expression and receptors are differentially modified in limbic foci by seizures

Results are consistent with the hypothesis that TRH may have an important role in the pathophysiology of epilepsy by modulating excitatory processes and in situ hybridization histochemistry in the hippocampus, hippocampus, and the amygdala.