Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.

Abstract

Chromosomal DNA from Streptococcus mutans strain MFe28 (serotype h) was cloned in the bacteriophage vector lambda L47.1. Two classes of recombinants were found which expressed glucosyltransferase activity in phage plaques: (i) gtfS, which expressed a glucosyltransferase synthesizing a water-soluble, dextranase-sensitive glucan, and (ii) gtfI, which… (More)

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@article{Gilpin1985CloningAE, title={Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.}, author={Martin L. Gilpin and Ryan Russell and Patrick F. Morrissey}, journal={Infection and immunity}, year={1985}, volume={49 2}, pages={414-6} }