Cloning and expression of three cecropin cDNAs from a mosquito cell line.

@article{Sun1999CloningAE,
  title={Cloning and expression of three cecropin cDNAs from a mosquito cell line.},
  author={Debin Sun and Eric D. Eccleston and Ann M Fallon},
  journal={FEBS letters},
  year={1999},
  volume={454 1-2},
  pages={147-51}
}
We have characterized full-length cDNAs encoding three isoforms of the antibiotic cecropin secreted by the C7-10 cell line from the mosquito, Aedes albopictus. The existence of two cecropin isoforms that differed from the previously described AalCecA was predicted by mass spectrometry and amino acid sequence analysis of peptides that eluted from reversed phase high performance liquid chromatography as a single peak just behind the previously described cecropin, AalCecA. Based on the amino acid… CONTINUE READING