Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.

  title={Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.},
  author={E L Katz and Charles B. Thompson and David A. Hopwood},
  journal={Journal of general microbiology},
  volume={129 9},
In two separate studies a BclI-generated DNA fragment coding for the enzyme tyrosinase, responsible for melanin synthesis, was cloned from Streptomyces antibioticus DNA into two SLP1.2-based plasmid vectors (pIJ37 and pIJ41) to generate the hybrid plasmids, designated pIJ700 and pIJ701, using S. lividans 66 as the host. The fragment (1.55 kb) was subcloned into the multicopy plasmid pIJ350 (which carries thiostrepton resistance and has two non-essential BclI sites) to generate four new plasmids… CONTINUE READING

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