Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.

@article{Katz1983CloningAE,
  title={Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.},
  author={E L Katz and Charles B. Thompson and David A. Hopwood},
  journal={Journal of general microbiology},
  year={1983},
  volume={129 9},
  pages={2703-14}
}
In two separate studies a BclI-generated DNA fragment coding for the enzyme tyrosinase, responsible for melanin synthesis, was cloned from Streptomyces antibioticus DNA into two SLP1.2-based plasmid vectors (pIJ37 and pIJ41) to generate the hybrid plasmids, designated pIJ700 and pIJ701, using S. lividans 66 as the host. The fragment (1.55 kb) was subcloned into the multicopy plasmid pIJ350 (which carries thiostrepton resistance and has two non-essential BclI sites) to generate four new plasmids… CONTINUE READING

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Genetics of Streptomyces glaucescens and regulation of melanin production

  • P.H.
  • 2nd International Symposium on Genetics of…
  • 1976
Highly Influential
9 Excerpts

Cloning of antibiotic resistance and nutritional genes from antibiotic producing species

  • A.D.
  • Journal of Bacteriology 151, 668-677.
  • 1982

Gene cloning in Streptomyces

  • J.C.
  • Current Topics in Microbiology and Immunol-
  • 1982

Membranebound penicillinases in gram-positive bacteria

  • J. B. K. NIELSEN
  • Journal of Biological Chemistry
  • 1982

Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction

  • D A.
  • Gene 20,
  • 1982

Bacillus lichenformis

  • W. C. Wu
  • 1981

Expression of the Streptomyces enzyme endoglycosidase H in Escherichia coli

  • C.
  • Journal of Biological Chemistry
  • 1981

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