Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum.

@article{Carr1986CloningAE,
  title={Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum.},
  author={Lucinda G. Carr and Paul L. Skatrud and Maurice E. Scheetz and Stephen W. Queener and Thomas D. Ingolia},
  journal={Gene},
  year={1986},
  volume={48 2-3},
  pages={257-66}
}
The isopenicillin N synthetase (IPS) gene from Penicillium chrysogenum was isolated from a recombinant bacteriophage lambda library using the Cephalosporium acremonium IPS (cIPS) gene as a heterologous hybridization probe. The protein coding region of the P. chrysogenum IPS (pIPS) gene was about 74% homologous to the cIPS gene, and the predicted amino acid sequences of the encoded proteins were about 73% homologous. Escherichia coli cells with the pIPS gene contained IPS activity whereas… CONTINUE READING

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