Cloning and expression of the gene coding for FtsH protease from Mycobacterium tuberculosis H37Rv.

@article{Anilkumar1998CloningAE,
  title={Cloning and expression of the gene coding for FtsH protease from Mycobacterium tuberculosis H37Rv.},
  author={Gopalakrishnapillai Anilkumar and Ms. Meena Chauhan and Parthasarathi Ajitkumar},
  journal={Gene},
  year={1998},
  volume={214 1-2},
  pages={
          7-11
        }
}
This study was aimed at the molecular cloning and expression of the gene coding for FtsH protease of Mycobacterium tuberculosis H37Rv (virulent). PCR on the genomic DNA of M. tuberculosis H37Ra (non-virulent) using the oligodeoxynucleotide primers, which were designed based on the codon usage pattern of M. tuberculosis and against the nucleotide (nt) sequence corresponding to two conserved domains of the FtsH protein of Escherichia coli, yielded a 363-bp product. The amino-acid sequence… CONTINUE READING
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