Cloning and expression of the cDNA sequence encoding the lysosomal glycosidase di-N-acetylchitobiase.

@article{Fisher1992CloningAE,
  title={Cloning and expression of the cDNA sequence encoding the lysosomal glycosidase di-N-acetylchitobiase.},
  author={Krishna J. Fisher and Nathan N. Aronson},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 27},
  pages={19607-16}
}
Di-N-acetylchitobiase (chitobiase) is a lysosomal glycosidase involved in the degradation of asparagine-linked glycoproteins. Previous studies have revealed that chitobiase is unique among lysosomal glycosidases in that it may not be expressed universally in mammals. In this study we have isolated full-length cDNA clones for human placenta and rat liver chitobiase. The cDNAs from both species encode a glycosylated polypeptide of approximately 40 kDa that displays chitobiase activity when… CONTINUE READING