Cloning and expression of the Zymomonas mobilis “Production of Ethanol” Genes in Lactobacillus casei

  title={Cloning and expression of the Zymomonas mobilis “Production of Ethanol” Genes in Lactobacillus casei},
  author={R. Shane Gold and Michael M. Meagher and Suxiang Tong and Robert W. Hutkins and Tyrrell Conway},
  journal={Current Microbiology},
This study describes the expression of the Zymomonas mobilis genes coding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) in Lactobacillus casei 686. To promote transcription, the promoter and ribosome binding site (RBS) from the Lactococcus lactis subsp. lactis- derived vector, pMGE36e, were inserted upstream of the pdc gene. The former sequences were positioned such that translation of pdc was coupled to translation of an 81-base pair open reading frame terminating within the… CONTINUE READING
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