Cloning and expression of squalene synthase cDNA from hot pepper (Capsicum annuum L.).

Abstract

We isolated and artificially expressed a cDNA clone of the Capsicum annuum squalene synthase (CASS) gene to elucidate the pattern of alternatively regulated two-branch point enzymes. The 1,674-bp CASS cDNA contained an open reading frame of 411 amino acids, yielding a predicted molecular mass of about 45 kDa. A deduced amino acid sequence comparison to… (More)

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