Cloning and expression of sheep DNA methyltransferase 1 and its development-specific isoform.

@article{Taylor2009CloningAE,
  title={Cloning and expression of sheep DNA methyltransferase 1 and its development-specific isoform.},
  author={Jane Taylor and Hannah R Moore and Nathalie Beaujean and John G. Gardner and Ian Wilmut and Richard Meehan and Lorraine Clarke Young},
  journal={Molecular reproduction and development},
  year={2009},
  volume={76 5},
  pages={501-13}
}
Unlike the mouse embryo, where loss of DNA methylation in the embryonic nucleus leaves cleavage stage embryos globally hypomethylated, sheep preimplantation embryos retain high levels of methylation until the blastocyst stage. We have cloned and sequenced sheep Dnmt1 and found it to be highly conserved with both the human and mouse homologues. Furthermore, we observed that the transcript normally expressed in adult somatic tissues is highly abundant in sheep oocytes. Throughout sheep… CONTINUE READING