• Biology
  • Published 2004

Cloning and expression of ophc2, a new organphosphorus hydrolase gene

@inproceedings{WUNingfeng2004CloningAE,
  title={Cloning and expression of ophc2, a new organphosphorus hydrolase gene},
  author={WUNingfeng and DENGMinjie and LIANGGuoyi and CHUXiaoyu and Yaobin and FANYunliu},
  year={2004}
}
The amino acid sequences of N-terminal and internal peptide of OPHC2, purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab, are determined. The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR. The ophc2 gene is 975 bp long with G+C content of 63%, comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD. The nucleotide… CONTINUE READING