Cloning and expression of new microRNAs from zebrafish

  title={Cloning and expression of new microRNAs from zebrafish},
  author={Wigard P. Kloosterman and Florian A Steiner and Eugene Berezikov and Ewart de Bruijn and Jos{\'e} van de Belt and Mark Verheul and Edwin Cuppen and Ronald H. A. Plasterk},
  booktitle={Nucleic acids research},
MicroRNAs (miRNAs) play an important role in development and regulate the expression of many animal genes by post-transcriptional gene silencing. Here we describe the cloning and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139 known miRNAs and 66 new miRNAs. For 65 known miRNAs and for 11 new miRNAs we also cloned the miRNA star sequence. We analyzed the temporal and spatial… CONTINUE READING
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