Corpus ID: 220995929

Cloning and expression of new apoptin fusion protein and its bioactivity.

@article{Jian2009CloningAE,
  title={Cloning and expression of new apoptin fusion protein and its bioactivity.},
  author={Zhao Jian and Xiao Wei and Fan Liqiang and Wang Fu-jun and L{\"u}JiaFeng and Yuan Qin-sheng and Liu Jianwen},
  journal={Food and Drug},
  year={2009},
  volume={11},
  pages={7-10}
}
Objective To clone and express apoptin-EC-SOD3 fusion protein and determine its bioactivity in vitro.Methods Apoptin gene was amplified by PCR and cloned into prokaryotic expression vector EC-SOD3-pET-28a.The constructed recombinant plasmid pET-28a-EC-SOD3-apoptin was transformed to E.coli BL21(DE3) for the expression under the induction of IPTG.The expressed protein was purified by Ni2+-NTA affinity chromatography and its activity was identified by MTT.Results PCR analysis proved that the… Expand
1 Citations
Organic chemicals from bioprocesses in China.
TLDR
Most of the products such as citric acid, xanthan and acrylamide etc., discussed in this paper have been in large-scale commercial production in China and are generally energy saving and environmentally benign. Expand