Corpus ID: 220995929

Cloning and expression of new apoptin fusion protein and its bioactivity.

  title={Cloning and expression of new apoptin fusion protein and its bioactivity.},
  author={Zhao Jian and Xiao Wei and Fan Liqiang and Wang Fu-jun and L{\"u}JiaFeng and Yuan Qin-sheng and Liu Jianwen},
  journal={Food and Drug},
Objective To clone and express apoptin-EC-SOD3 fusion protein and determine its bioactivity in vitro.Methods Apoptin gene was amplified by PCR and cloned into prokaryotic expression vector EC-SOD3-pET-28a.The constructed recombinant plasmid pET-28a-EC-SOD3-apoptin was transformed to E.coli BL21(DE3) for the expression under the induction of IPTG.The expressed protein was purified by Ni2+-NTA affinity chromatography and its activity was identified by MTT.Results PCR analysis proved that the… Expand
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Organic chemicals from bioprocesses in China.
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